TABLE 2.
Rate of identification of B. melitensis 16M, cultivated under different conditions and inactivated with the solvent mixture before MALDI-TOF MS
| Strain | Mediuma | Incubation conditions | No. identified/ no. analyzed (%)b |
|---|---|---|---|
| 16M | COS-B | 24 h | 2/2 (100) |
| 16M | COS-B | 48 h | 10/11 (91) |
| 16M | COS-B | 48 h, +5% CO2 | 2/2 (100) |
| 16M | COS-B | 72 h | 2/2 (100) |
| 16M | COS-B | 96 h | 2/2 (100) |
| 16M | COS-B | 96 h, +5% CO2 | 2/2 (100) |
| 16M | COS-O | 48 h | 2/2 (100) |
| 16M | MHB | 48 h | 3/4 (75) |
| 16M | MHF | 48 h | 3/4 (75) |
| 16M | BAS | 48 h | 6/6 (100) |
| 16M | BBA | 48 h | 4/4 (100) |
| 16M | CHOC | 48 h | 4/4 (100) |
| 16M | TS | 48 h | 2/2 (100) |
| 16M | TSA-S | 48 h | 3/4 (75) |
| bIN1983c | COS-B | 48 h | 7/7 (100) |
COS-B, Columbia agar with 5% sheep blood (bioMérieux); COS-O, Columbia agar with 5% sheep blood (Oxoid); MHB, Mueller-Hinton agar with 5% sheep blood (Bio-Rad); MHF, Mueller-Hinton agar with 5% horse blood and β-NAD (Bio-Rad); BAS, Brucella agar with 5% sheep blood, hemin, and vitamin K1 (Becton, Dickinson); BBA, Brucella blood agar (bioMérieux); CHOC, chocolate agar with vitox (Oxoid); TS, homemade trypticase soy agar; TSA-S, trypticase soy agar with 5% sheep blood (Becton, Dickinson).
Shown are the numbers of successful identifications/number of spectra analyzed, as well as the rate of success.
bIN1983, strain 16M plus pMR10-GFP.