Fitted ROC curves of VLP- and NS1-MAC-ELISAs on JEV-infected (A) and WNV-infected (B) human sera. Assay performances between JEV VLP- and NS1-MAC-ELISAs on the target JEV-infected serum panel (A) or between WNV VLP- and NS1-MAC-ELISAs on the target WNV-infected serum panel (B) against the control panel, including DENV, YFV, Zika, HTN, CHIKV, and negative panels, were compared.