TABLE 1.

Test accuracy of MAC-ELISA using VLP and/or NS1 antigens^a

Control panel by disease panel	Antigen	ELISA result	True status		AUC (95% CI)	% sensitivity (95% CI)	% specificity (95% CI)
			Disease	No disease
JEV
Other groupsb	JEV VLP	Positive	16	5	1.00 (1.00–1.00)	100.00 (79.41–100.00)	92.54 (83.44–97.53)
		Negative	0	62
	JEV NS1	Positive	16	0	1.00 (1.00–1.00)	100.00 (79.41–100.00)	100.00 (94.64–100.00)
		Negative	0	67
	JEV VLP and NS1	Positive	16	0	NAc	100.00 (79.41–100.00)	100.00 (94.64–100.00)
		Negative	0	67
WNV and other groups	JEV VLP	Positive	16	23	0.99 (0.99–1.00)	100.00 (79.41–100.00)	82.44 (74.83–88.53)
		Negative	0	108
	JEV NS1	Positive	16	41	0.99 (0.96–1.01)	100.00 (79.41–100.00)	68.70 (60.02–76.52)
		Negative	0	90
	JEV VLP and NS1	Positive	16	13	NA	100.00 (79.41–100.00)	90.08 (83.63–94.61)
		Negative	0	118
WNV
Other groups	WNV VLP	Positive	64	0	1.00 (1.00–1.00)	100.00 (94.40–100.00)	100.00 (94.64–100.00)
		Negative	0	67
	WNV NS1	Positive	64	2	0.99 (0.98–1.01)	100.00 (94.40–100.00)	97.01 (89.63–99.63)
		Negative	0	65
	WNV VLP and NS1	Positive	64	0	NA	100.00 (94.40–100.00)	100.00 (94.64–100.00)
		Negative	0	67
JEV and other groups	WNV VLP	Positive	64	8	0.99 (0.98–1.00)d	100.00 (94.40–100.00)	90.36 (81.89–95.75)
		Negative	0	75
	WNV NS1	Positive	64	16	0.89 (0.83–0.94)	100.00 (94.40–100.00)	80.72 (70.59–88.56)
		Negative	0	67
	WNV VLP and NS1	Positive	64	8	NA	100.00 (94.40–100.00)	90.36 (81.89–95.75)
		Negative	0	75

^aAccuracy was defined as the ability to distinguish the disease panel (JEV or WNV serum panels) from the control panel using the positive-cutoff criterion (P/N ≥ 3.0) as the evidence of infection.

^bOther groups include DENV, YFV, Zika, HTN, CHIKV, and negative serum panels.

^cNA, not available.

^dP < 0.05.