FIG. 2.
Single-cell mRNA profiling analysis of unsorted neurosphere cells. (A) Forty-eight-dimensional mRNA profiling data from passage 2 unsorted neurosphere cells were compressed to 2 dimensions by nMDS. Axes for the 2 dimensions are labeled as nMDS_1 and nMDS_2. Mclust was then used to cluster cells based on the mRNA profile of 48 genes. Three clusters were derived—US1, US2, and US3. (B) Heatmap showing the mRNA profile of the different clusters. Each row represents one cell, and each column represents one gene. Genes under the P<0.05 section are genes that show a statistically significant change in expression in cluster US1 compared to clusters US2 and US3 combined. (C) Bar plots showing a log2-fold change in gene expression for genes that show significant change in expression (P<0.05) in a cluster in comparison with the remaining clusters combined. nMDS, nonmetric multidimensional scaling; US1, unsorted 1.