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. 2012 Jul 26;21(18):3351–3362. doi: 10.1089/scd.2012.0232

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 4. — Characterization of FSC/SSC-sorted populations. (A) Average cell size of FSC/SSC^high and FSC/SSC^low cells (mean±SD; n=22 and n=15 for FSC/SSC^high and FSC/SSC^low cells respectively; *P≤0.05). (B) Correlation of neurosphere formation to cell size range (mean±SD; n=4; *P≤0.05). (C) Fluorescence intensity of 57 FSC/SSC^high and 1564 FSC/SSC^low cells was recorded after phosphacan staining (mean±SD; ***P≤0.001). (D) Fluorescence intensity distribution of phosphacan staining in FSC/SSC^high and FSC/SSC^low cells. (E) Percentage of Lewis-X- positive and negative cells in the FSC/SSC^high and FSC/SSC^low populations (mean±SD; n=4; **P≤0.01). (F) Fluorescence intensity distribution of Lewis-X in the FSC/SSC^high and FSC/SSC^low populations. Cells were stained with FITC-conjugated anti-Lewis-X antibody, and the fluorescence intensity of 1000 cells per population was recorded. Passage 2 cells were used for the experiments. FITC, fluorescein isothiocyanate.