FIG. 6.

Single-cell mRNA profiling analysis of FSC/SSC^high and FSC/SSC^low cells. (A) Forty-eight-dimensional mRNA profiling data from passage 2 FSC/SSC^high and FSC/SSC^low cells were compressed to 2 dimensions by nMDS. Axes for the 2 dimensions are labeled as nMDS_1 and nMDS_2. Mclust derived 3 clusters from FSC/SSC^high (filled circles) and FSC/SSC^low (open circles) cells—cluster S1 (red), S2 (green), and S3 (blue). (B) Heatmap showing the mRNA profile of the different clusters. Each row represents 1 cell, and each column represents 1 gene. Genes under the P<0.05 section are genes that show a statistically significant change in expression in cluster S1 compared to clusters S2 and S3 combined. (C) Bar plots showing log₂-fold change in gene expression for genes that show significant change in expression (P<0.05) in a cluster in comparison with the remaining clusters combined. S, sorted.