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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Exp Hematol. 2015 Oct 21;44(2):106–115. doi: 10.1016/j.exphem.2015.10.003

Figure 2. Evaluation of vector RNA internalization in transduced HeLa and human CD34+ cells.

Figure 2

(a) We determined vector RNA amounts in the cells for 5–60 minutes after viral exposure. In HeLa cells, vector RNA amounts rapidly increased and then plateaued at 30 minutes. (b) In human CD34+ cells, vector RNA amounts gradually increased over 60 minutes. (c) We calculated vector RNA ratios of CD34+ cells to HeLa cells at same MOI 5, which resulted in low vector RNA ratios (0.28–0.49) at 5–30 minutes and around 1 of vector RNA ratio at 60 minutes. (d) When we calculated the ratios of vector RNA amounts at high to low MOIs, the vector RNA ratios were slightly less than 10-fold in both cells over 60 minutes. Equivalent or slightly lower ratios were observed in human CD34+ cells, compared to HeLa cells. These data suggest that similar amounts of vector genome can enter human CD34+ cells even with slower internalization, as compared to HeLa cells.