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. Author manuscript; available in PMC: 2017 Jan 28.
Published in final edited form as: Cell. 2016 Jan 21;164(3):365–377. doi: 10.1016/j.cell.2016.01.002

Figure 2. Tumor-associated GzmB-expressing T cells are unconventional.

Figure 2

(A) Flow cytometric analysis of NK1.1 expression in TCR−GzmB+ (green), TCRβ+GzmB+ (red) and TCRδ+GzmB+ (blue) cells in wild-type (WT) and 8-week-old PyMT mice.

(B) Flow cytometric analysis of NK1.1 expression and CD1d/PBS-57 tetramer reactivity in TCRβ+ lymphocytes (left), and GzmB expression in TCRβ+NK1.1+CD1d/PBS-57− cells (red) and TCRβ+CD1d/PBS-57+ cells (purple) from pooled mammary glands of 8-week-old PyMT mice. Grey histogram indicates fluorescence minus one (FMO) control.

(C) Flow cytometric analysis of CD4 and CD8α expression in TCRβ+NK1.1+CD1d/PBS-57− lymphocytes from pooled mammary glands of 8-week-old PyMT mice. Numbers in quadrants indicate percentage of cells.

(D) Flow cytometric analysis of PD-1 and NK1.1 expression in TCRβ+CD8α+ cells from pooled mammary glands of WT, 8- and 20-week-old PyMT mice. Numbers in quadrants indicate percentage of cells.

(E) Flow cytometric analysis of CD28, ICOS and CD5 expression among CD8α−NK1.1+ (dark orange), CD8α+NK1.1+ (light orange), CD8α+NK1.1−PD-1− (dotted grey) and CD8α+PD-1+ (black) T cell populations from pooled tumors of 20- to 24-week-old PyMT mice. Solid grey line in histograms indicates fluorescence minus one (FMO) control for each population.

See also Figure S2.