Figure 7. IL-15 regulates ILC1l and ILTC1 generation impacting on tumor growth.

(A) Flow cytometric analyses of CD49a and CD49b expression in TCR⁻NK1.1⁺ cells from pooled tumors of Nfil3^−/− PyMT and wild-type PyMT mice. Numbers in plots indicate percentage of CD49a^hiCD49b⁻ (ILC1l) and CD49a⁻CD49b⁺ (cNK) cells in their respective gates. Data are representative of three independent experiments.

(B) Flow cytometric analysis of CD49a and GzmB expression in CD45⁺ leukocytes from pooled tumors of Nfil3^−/− PyMT and wild-type PyMT mice. Numbers in plots indicate percentage of CD49a^hiGzmB⁺ cells. Data are representative of three independent experiments.

(C) Total tumor burden of Nfil3^−/− PyMT and wild-type PyMT mice monitored between 8 and 15 weeks of age (n = 3–6).

(D) Flow cytometric analysis of CD122 expression in the indicated cell populations isolated from pooled mammary glands of 8-week-old PyMT mice. Data are representative of five independent experiments.

(E) Flow cytometric analysis of CD49a and GzmB expression in CD45⁺ leukocytes isolated from pooled mammary glands of 8-week-old Il15^−/− PyMT and wild-type PyMT mice. Numbers in plots indicate percentage of CD49a^hiGzmB⁺ cells.

(F) Total tumor burden of Il15^−/− PyMT and wild-type PyMT mice monitored between 8 and 15 weeks of age (n = 14–17).

(G) Flow cytometric analysis of CD49a and GzmB expression in CD45⁺ leukocytes isolated from pooled mammary glands of 8-week-old IL-15^TgPyMT and wild-type PyMT mice. Numbers in plots indicate percentage of CD49a^hiGzmB⁺ cells.

(H) Total tumor burden of IL-15^TgPyMT and wild-type PyMT mice monitored between 8 and 15 weeks of age (n = 11–15).

(C, F, H) Error bars represent the mean ± SEM. Two-way ANOVA was used for statistical analysis. n.s. = not significant, *P<0.05, **P<0.01.

See also Figure S7.