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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Am J Transplant. 2015 Nov 9;16(2):672–678. doi: 10.1111/ajt.13483

Figure 1. Kinetics of γ-IFN production by naïve and memory T cells.

Figure 1

Naïve (CD44−) and memory (CD44+) T cells were isolated from the spleen of BALB/c mice 40 days after placement of a B6 skin allograft. The T cells were cultured for various periods of time with irradiated B6 antigen-presenting cells and the frequencies of activated cells producing γ-interferon (γ-IFN) were assessed by ELISPOT. The results are expressed as numbers of γ-IFN spots per million T cells and represent four to six mice tested individually±standard deviation.