Skip to main content
. Author manuscript; available in PMC: 2016 Oct 8.
Published in final edited form as: Cell. 2015 Oct 1;163(2):493–505. doi: 10.1016/j.cell.2015.08.033

Figure 1. A super-resolution imaging and analysis platform.

Figure 1

(A) Tissues were dissected, fixed for immunohistochemical labeling, postfixed, dehydrated, and embedded in epoxy resin. Ultrathin sections were cut, arrayed on glass coverslips, and etched to expose fluorophores for STORM imaging. Individual serial sections were imaged and aligned to generate 3D reconstructions. (B) STORM maximum intensity projection of a volume (2.3 × 105 μm3) of the mouse IPL containing an On-Off DSGC (blue) amidst presynaptic (magenta) and gephyrin (green) clusters imaged using the platform. (C) An enlarged image of synapses in a small region (1 μm thickness) of the IPL. For comparison, the corresponding conventional image of the upper right portion (to the right of the dashed line) is presented. See also Figure S1 and Figure S2.