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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Proc SPIE Int Soc Opt Eng. 2015 Mar 11;9336:93360S. doi: 10.1117/12.2081319

Figure 3.

Figure 3

CINCH and confocal imaging of a sample with two layers of 1 μm beads separated by ca. 50 μm, taken with a 20x Nikon objective. (a-c) The CINCH holograms are shown as log(intensity). (d-f) Hologram phase maps, (g-i) CINCH images and (j-l) confocal images are displayed in a linear scale with intensity bars indicating the relative intensity of each image. Each column of images results from image capture with the designated sample plane at the focal plane of the objective. Sample plane 1 contains the top layer of beads, sample plane 3 contains the bottom layer and sample plane 2 is equidistant between the two layers. The CINCH phase images contain the depth dependent phase information derived from the CINCH holograms and the reconstructed CINCH images show the complex holograms propagated to the best focal plane. From Reference 17.