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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Proc SPIE Int Soc Opt Eng. 2015 Mar 11;9336:93360S. doi: 10.1117/12.2081319

Figure 4.

Figure 4

Two different object planes, separated by 25 μm, of a fluorescent pollen grain sample studied by four microscope methods with a 60× Nikon CFI objective. The lobed pollen grain is in focus in plane 1 but out of focus in plane 2, while the spiked pollen grain is in focus in plane 2 but not in plane 1. From Reference 17.