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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Clin Immunol. 2011 Jan 15;138(3):274–281. doi: 10.1016/j.clim.2010.12.002

Figure 5. Reactivity of NK cell subsets can be predicted based on KIR ligand group typing.

Figure 5

KIR ligand typing of donor cells was done using a single-nucleotide polymorphism assay and followed by prediction of NK cell licensing. Single KIR+ NK cell subsets were gated using monoclonal antibodies against KIRs, and NK cell degranulation was measured using a CD107 marker. Reactivity of NK cell subsets toward target cells ectopically expressing their ligands was determined. Shown are the reactivity of the A) educated (left panel) and uneducated (right panel) KIR2DL1+ NK cell subsets toward 721.221 cells expressing the ligand Cw6 or non-ligand Cw7; * p value <0.05; and the B) educated (left) and uneducated (right) KIR2DL2/2DL3+ NK cell subsets toward 721.221 cells expressing the non-ligand Cw6 or ligand Cw7; * p value <0.05.