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RNA was isolated from human OSA cell lines, reverse transcribed, and assayed using qPCR. OSA marker expression was scored based on mean qPCR Ct values as follows: - >30; +, 25– 30; ++, 20–25; +++, <20.
| MG63 | Saos2 | SJSA1 | |
|---|---|---|---|
| ALPL | + | + + | + + |
| BGLAP | − | + | − |
| IBSP | + | − | + |
| DMP1 | − | + | − |
| FGF23 | − | − | − |
| MEPE | − | + | − |
| OPG | + + + | + + | + |
| PDPN | + + | − | + + |
| PHEX | + | + | − |
| SOST | − | − | − |
| TNFSF11 | − | + | − |
| β-ACTIN | + + + | + + + | + + + |
| GAPDH | + + + | + + + | + + + |
