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. Author manuscript; available in PMC: 2016 Dec 7.
Published in final edited form as: Mol Pharm. 2015 Oct 23;12(12):4488–4497. doi: 10.1021/acs.molpharmaceut.5b00645

Figure 1.

Figure 1

(a-c) Representative confocal microscopy z-slice images of cells expressing importin-4 (green) with the nuclei stained with DAPI (blue) 6 h after a pulse-transfection with PEI polyplexes (a), H3-targeted polyplexes (b), or sH3 polyplexes (c). Polyplexes are in red; arrows indicate regions of colocalization between polyplexes and importin-4. The scale bar (shown in a) = 10 μm. The cell borders were outlined in white by comparison with the corresponding phase images by using Zen software. (d) Mander's coefficients quantifying colocalization between polyplexes and importin-4 from confocal microscopy images taken at different times post-transfection, performed by Volocity Image Analysis Software. Untargeted PEI polyplexes (black), H3-targeted polyplexes (dark gray), and sH3 polyplexes (light gray) were transfected in CHO cells and colocalization was analyzed at various times post-transfection. Each data point represents the mean ± SE with a minimum of 80-100 polyplexes analyzed. The dotted line indicates mitosis. *Indicates a statistically significant difference from PEI polyplexes at the same time point (P < 0.05). **Indicates a statistically significant difference from the previous time point for the given polyplex (P < 0.05).