Fig. 6.

Activation of the NF-κB pathway by VRL. Both the p65 and the p50 subunits were determined in the nuclear extracts of HUVECs (normalized to lamin-B). IκBa proteins were determined in the cytosol and normalized to β-actin. The presented values express the protein levels of NF-κB units normalized to the respective housekeeping protein before and after VRL treatment; for IκBa, values are for pIκBa and IκBa, respectively. The last two lines correspond to pIκΒa/ IκΒa ratio before and after VRL treatment at each time point. The upper line corresponds to pIκBa and the lower line to IκBa. Representative figures of two independent experiments are shown. HUVEC, human umbilical vein endothelial cell; NF-κB, nuclear factor-κB; VRL, vinorelbine.