Figure 3.
Analysis of the patient with the smallest EcoRI fragment of 10 kb. (a) DNA was digested with EcoRI (E) and EcoRI/BlnI (B), separated by pulsed-field gel electrophoresis and hybridized with probe p13E-11. 4q35-located alleles are resistant to BlnI, and 10q26-located alleles are sensitive to BlnI. Two 4q-type alleles of 10 kb (arrow) and 50 kb; two 10q-type alleles of 42 kb and 156 kb; (b) The same DNA was digested with Hind III (H) and hybridized with probes 4qA and 4qB. 4q-type allele of 10 kb (arrow) was identified as 4qA variant and 50 kb-allele was 4qB variant. M: MidRange PFG marker Y: Y chromosome.