Table 2.
Oligonucleotide primers for real-time PCR used in the study
| Primers | Prime sequences (5’-3’) | Product length (bp) |
|---|---|---|
| nNOS | ||
| Forward | TGCGAACGTACGAAGTGACCA | 149 |
| Reverse | GCAGTGTCTGTCCGCGCTTA | |
| iNOS | ||
| Forward | ACGTGCGTTACTCCACCAACAA | 109 |
| Reverse | CGGATGAGCTGAGCATTCCA | |
| eNOS | ||
| Forward | GCTGTCTGCATGGACCTGGA | 119 |
| Reverse | TCCACGATGGTGACTTTGGCTA | |
| β-actin | ||
| Forward | TGGCACCCAGCACAATGAA | 186 |
| Reverse | CTAAGTCATAGTCCGCCTAGAAGCA |
nNOS: Neuronal nitric oxide synthase; iNOS: Inducible nitric oxide synthase; eNOS: Endothelial NOS; PCR: Polymerase chain reaction.