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. 2015 Sep 5;128(17):2346–2353. doi: 10.4103/0366-6999.163375

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Copyright: © 2015 Chinese Medical Journal

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Western blotting analysis of myocardium Wnt3a. The Wnt3a (37 kDa) (a), was analyzed by Western blot with specific Wnt3a antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; ^†P < 0.05 versus ischemia-reperfusion; ^‡P < 0.05 versus sevoflurane preconditioning (one-way ANOVA); Western blot analysis of myocardium p-GSK3β. The p-GSK3β (47 kDa) (b) was analyzed by Western blot with specific p-GSK3β antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; ^†P < 0.05 versus ischemia-reperfusion; ^‡P < 0.05 versus sevoflurane preconditioning (one-way ANOVA); Western blot analysis of myocardium β-catenin. The β-catenin (c) was analyzed by Western blot with specific β-catenin antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; ^†P < 0.05 versus ischemia-reperfusion; ^‡P < 0.05 versus sevoflurane preconditioning (one-way ANOVA).