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. 2015 Sep 5;128(17):2346–2353. doi: 10.4103/0366-6999.163375

Figure 4.

Figure 4

Western blotting analysis of H9C2 cells. The Wnt3a (37 kDa) (a), was analyzed by Western blot with specific Wnt3a antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; †P < 0.05 versus ischemia-reperfusion; ‡P < 0.05 versus sevoflurane preconditioning (one-way ANOVA); GSK3β: Glycogen synthase kinase 3β; Western blot analysis of H9C2 cells. The p-GSK3β (47 kDa) (b) was analyzed by Western blot with specific p-GSK3β antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; †P < 0.05 versus ischemia-reperfusion; ‡P < 0.05 versus sevoflurane preconditioning (one-way ANOVA); GSK3β: Glycogen synthase kinase 3β; Western blot analysis of H9C2 cells. The β-catenin (c) was analyzed by Western blot with specific β-catenin antibody at the end of reperfusion. Three hearts were used in each group. Data are reported as means ± standard deviation. *P < 0.05 versus S; †P < 0.05 versus ischemia-reperfusion; ‡P < 0.05 versus Sevoflurane preconditioning (one-way ANOVA).