Figure 5.
Expression analysis of the genes selected by a microarray analysis (HUES8>HUES6) in PiPSCs and FiPSCs.(A) A quantitative RT-PCR analysis of the GATA6 and GREM1 genes, in which the expression was higher in HUES8 than in HUES6 on both GPL3921 and GPL570, in FiPSCs and PiPSCs. (B) A quantitative RT-PCR analysis of GATA3, GATA4, and PAX6 genes, in which the expression was higher in HUES8 than HUES6 on only GPL3921, in FiPSCs and PiPSCs. (C) A quantitative RT-PCR analysis of SOX2, GAL, SOX17 genes, in which expression was higher in HUES8 than HUES6 on only GPL570, in FiPSCs and PiPSCs. The data are expressed as the genes-to-GAPDH ratio, with that of FiPSCs arbitrarily set at 1 (n = 3).