Table 1.
Primer Sequences of Quantitative RT-PCR
| Gene | Sequences (5′ to 3′) |
|---|---|
| ESC-specific genes | |
| OCT3/4 | Forward: GAAACCCACACTGCAGCAGA Reverse: TCGCTTGCCCTTCTGGCG |
| SOX2 | Forward: GGGAAATGGGAGGGGTGCAAAAGAGG Reverse: TTGCGTGAGTGTGGATGGGATTGGTG |
| NANOG | Forward: CTCAGCTACAAACAGGTGAAGAC Reverse: TCCCTGGTGGTAGGAAGAGTAAA |
| REX1 (ZFP42) | Forward: CAGATCCTAAACAGCTCGCAGAAT Reverse: GCGTACGCAAATTAAAGTCCAGA |
| TERT | Forward: CGTACAGGTTTCACGCATGTG Reverse: ATGACGCGCAGGAAAAATGT |
| GAL | Forward: AAACAATATCATGCGCACAATCA Reverse: GGGCACCGGCCTCTTT |
| LEFTY2 | Forward: GCTCAGATGCTGAGCTCTAGTAGGA Reverse: GAAACTCCCAGCTGAAAATGTGT |
| GAPDH | Forward: CCACTCCTCCACCTTTGACG Reverse: ATGAGGTCCACCACCCTGTT |
| Endoderm-specific genes | |
| SOX17 | Forward: AGCCAAGGGCGAGTCCCGTA Reverse: GCCTTCCACGACTTGCCCAGC |
| CXCR4 | Forward: CTGCACCTGTCAGTGGCCGA Reverse: TTGGTGGCGTGGACGATGGC |
| WNT3 | Forward: TCCTCGGCGCCTCTTCTAAT Reverse: CTGTGAGCCCAGAGATGTGT |
| CD117 (KIT) | Forward: ATTCAAGCACAATGGCACGG Reverse: AAGGAGTGAACAGGGTGTGG |
| GATA3 | Forward: CTGGCTCGCAGAATTGCA Reverse: TGGGTACGGCAGAATAAAACG |
| GATA4 | Forward: GTTTTTTCCCCTTTGATTTTTGATC Reverse: AACGACGGCAACAACGATAAT |
| GATA6 | Forward: GGATTGTCCTGTGCCAACTGT Reverse: GGTTCACCCTCGGCGTTT |
| HNF1B | Forward: AACCACCGAAGAGGAAGCAA Reverse: TCGCATCAGTTTGTTCGATGA |
| HNF4A | Forward: CACCTGATGCAGGAACATATGG Reverse: CTGTCCGTTGCTGAGGTGAGT |
| AFP | Forward: GTTGCCAACTCAGTGAGGACAA Reverse: TGATACATAAGTGTCCGATAATAATGTCA |
| Pancreas-specific genes | |
| PDX1 | Forward: GATACTGGATTGGCGTTGTTTG Reverse: TCCCAAGGTGGAGTGCTGTAG |
| HNF6 | Forward: TGCGCAACCCCAAACC Reverse: TCCACATCCTCCGGAAGGT |
| NKX6.1 | Forward: GCCTGTACCCCTCATCAAGGA Reverse: AAGTGGGTCTCGTGTGTTTTCTC |
| INS | Forward: ACGAGGCTTCTTCTACACACCC Reverse: TCCACAATGCCACGCTTCTGCA |
| PAX6 | Forward: GCTTCACCATGGCAAATAACCT Reverse: GGCAGCATGCAGGAGTATGA |
| SOX9 | Forward: CCCATGTGGAAGGCAGATG Reverse: GAAGGTTAACTGCTGGTGTTCTGA |
| Genes selected by a microarray analysis | |
| GREM1 | Forward: CCCCCCGCCAGACAAG Reverse: TTGCACCAGTCTCGCTTCAG |
| EGR1 | Forward: TTTCACGTCTTGGTGCCTTTT Reverse: TCCCTCACAATTGCACATGTC |
| NLRP2 | Forward: CTTTGAGGAAACCACTGTGCAA Reverse: AACTGAACGGAGGGATGGAA |
| SMC3 | Forward: TTGCTCTGATTTTTGCCATTCA Reverse: CATCCAGAGCCTGGTCAATTTC |
OCT3/4, octamer-binding transcription factor 3/4 [also known as pituitary-specific positive transcription factor 1 octamer-binding transcription factor 1 Unc-86 (POU) class 5 homeobox 1(POU5F1)]; SOX2: sex-determining region Y box 2; REX1, reduced expression protein 1 (Zinc finger protein 42; ZFP42); TERT, telomerase reverse transcriptase; GAL:, galanin/galanin-message-associated peptide (GMAP) pre-propeptide; LEFTY2, left-right determination factor 2; GAPDH, glyceraldehyde 3-phospahte dehydrogenase; CXCR4, chemokine C-X-C motif receptor 4; WNT3, wingless-type MMTV integration site family, member 3; CD117, cluster of differentiation 117 (also known as v-kit Hardy–Zuckerman 4 feline sarcoma viral oncogene homolog, KIT); GATA3, guanine–adenine–thymine–adenine-binding protein 3; HNF1B, hepatocyte nuclear factor 1B; AFP, αfeto-protein; PDX1, pancreatic and duodenal homeobox 1; NKX6.1, NK6 homeobox 1; INS, insulin; PAX6, paired box 6; GREM1, Gremlin 1 [also known as differential screening-selected gene aberrative in neuroblastoma (DAN) family bone morphogenetic protein (BMP) antagonist]; EGR1, early growth response 1; NLRP2, nucleotide-binding domain and leucine-rich repeat containing gene (NLR) family, pyrin domain containing 2; SMC3, structural maintenance of chromosomes 3.