Skip to main content
. 2014 Mar 24;7(1):37–47. doi: 10.3727/215517914X680047

Figure 2.

Figure 2

In vitro islet function and survival of intraislet ECs in adipose MSC precultured islets. (A) Insulin release at 36 mg/dl and 360 mg/dl glucose of 10 replicates of triplicate islets precultured for 3 days with adipose MSCs (white bars) or without MSCs (black bars); *p < 0.05 versus absence of MSCs at the same glucose concentration (two-way ANOVA with Bonferroni post hoc test, n = 4). (B) Preculturing islets with adipose MSCs has no effect on islet insulin content. Insulin content of islets precultured alone (black bar) or with adipose MSCs (white bar) for 3 days; p > 0.2 (Student’s t test, n = 6). (C–E) Preculturing islets with adipose MSCs does not have any angiogenic effect in vitro. (C, D) Immunostaining of endothelial cells (ECs) with CD31 antibodies in islets precultured alone (C) or precultured with adipose MSCs (D) for 3 days. Original magnification: 400×. Scale bars: 25 µm. (E) Vascular density of islets precultured alone (black bar) or with adipose MSCs (white bar) for 3 days; p > 0.05 (Student’s t test, n = 64–74 islet sections from four mice in each culture group).