Fig. 3.

Canalicular protein trafficking is impaired in VL-17A cells. VL-17A cells expressing pIgA-R were treated in the absence or presence of 50 mM ethanol (EtOH) for 24 h as indicated. Live cells were basolaterally labeled for pIgA-R with specific antibodies for 20 min at 4 °C. After washing away excess antibodies, antibody-antigen complexes were chased for 0 (a, d) or 15 min (b, c, e) at 37 °C in the continued absence or presence ethanol. Cells were fixed and permeabilized and labeled with secondary antibodies to detect the transcytosed proteins. Bar 10 μm. Arrows are marking the canalicular/apical compartment in control cells