Fig. 5.

Dynein and dynactin colocalize with stalled transcytosing proteins. A WIF-B cells were treated in the absence or presence of 50 mM ethanol (EtOH) for 72 h and labeled for dynein intermediate chain (dyn) and p150 as indicated. In the higher magnification images (c and f), arrows mark discrete puncta observed only in ethanol-treated cells. Asterisks mark selected bile canaliculi. Bar 10 μm. B WIF-B cells were treated with 50 mM ethanol (EtOH) for 72 h. APN was continuously basolaterally labeled for 45 min and cells processed for immunodetection of trafficked (tr) APN and steady state (ss) dynein (dyn) (a–c) or p150 (d–f). Merged images are shown in c and f. Arrows point to regions of colocalization. Bar 10 μm