. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: Mol Cell Biochem. 2014 Aug 23;397(0):223–233. doi: 10.1007/s11010-014-2190-4

Table 1.

Ethanol treatment does not alter dynein/dynactin membrane attachment

	% Distribution
	Dynein		p150		α-Tubulin
	Con	EtOH	Con	EtOH	Con	EtOH
P₂	0.0 ± 0.0	0.0 ± 0.0	19.5 ± 7.1	18.7 ± 9.5	4.8 ± 4.8	1.8 ± 1.8
S₃	76.1 ± 6.2	81.0 ± 19.0	41.8 ± 5.9	50.8 ± 6.9	93.5 ± 6.5	98.2 ± 1.8
P₃	23.9 ± 16.2	19.0 ± 19.0	38.8 ± 1.5	30.5 ± 10.2	1.7 ± 1.7	0.0 ± 0.0

Membrane fractions (see “Materials and methods” section) were immunoblotted for dynein intermediate chain, p150 or α-tubulin as indicated. Relative protein distributions in the cytosol (S3) and the heavy (P2) and light (P3) membrane fractions were determined by densitometric analysis of immunoreactive bands. Values are expressed as the mean ± SEM and were determined from at least three independent experiments