. Author manuscript; available in PMC: 2016 Jan 31.

Published in final edited form as: Mol Cell Biochem. 2014 Aug 23;397(0):223–233. doi: 10.1007/s11010-014-2190-4

Table 2.

Dynein binds microtubules more tightly in ethanol-treated cells

	Percent Microtubule Associated
	Con	EtOH
Dynein	16.7 ± 1.5	100.0 ± 0.0^*
p150	25.5 ± 6.1	17.5 ± 2.6

Microtubules were polymerized and pelleted from cytosolic extracts (see “Materials and methods” section) from control or ethanol (EtOH) treated cells. The supernatant and microtubule pellet were immunoblotted for dynein intermediate chain and p150. The percent of pelleted protein was determined by densitometric analysis of immunoreactive bands and values corrected for volume. Values are expressed as the mean ± SEM and were determined from at least three independent experiments.

p ≤ 0.001