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. 2015 Aug 26;8(1-2):9–23. doi: 10.3727/215517915X689038

Figure 4.

Figure 4

Gene expression profiling of ESC-like colonies. (a) RT-PCR analysis performed to detect the expression of endogenous genes [OCT3/4, sex-determining region Y box 2 (SOX2), Krüppel-like factor 4 (KLF4), NANOG, and ALP] in HDDPC-iPSCs, HDFa (negative control), and PA-1 (positive control). Expression of OCT3/4 and SOX2 mRNA from the exogenous construct was also assessed using primer sets βA-1/OCT3/4-AS (for OCT3/4) and βA-1/SOX2-RV (for SOX2). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. −RT indicates the negative control (PCR with no RT product). M, 100-bp ladder markers. (b) PCR analysis of genomic DNA to detect the presence of integrated transgenes using the primer sets β-gl-1S/OCT3/4-AS (for pCXLE-hOCT3/4-shp53) and β-gl-1S/SOX2-RV (for pCXLE-hSK). As positive controls, plasmid DNA (5 ng) was concomitantly subjected to PCR and loaded.