Table 1.

Primer Sets Used for PCR Analysis

Target mRNAor DNA	Primer Name: Sequence (5′–3′)	Size (bp)	Reference
Endogenous OCT3/4 mRNA	OCT3/4-S (forward): ATT TCA CCA GGC CCC CGG CT OCT3/4-AS (reverse): GCT GAT CTG CTG CAG TGT GGG T	822	16
Endogenous SOX2 mRNA	SOX2-S (forward): AGG ACC AGC TGG GCT ACC CG SOX2-AS (reverse): GGC GCC GGG GAG ATA CAT GC	320	29
Endogenous KLF4 mRNA	KLF4-S (forward): CGT GCT GAA GGC GTC GCT GA KLF4-AS (reverse): GGG TGC ACG AAG AGA CCG CC	173	10
Endogenous NANOG mRNA	N-S (forward): CCT CCA GCA GAT GCA AGA ACT C N-AS (reverse): GTA AAG GCT GGG GTA GGT AGG TG	172	46
Endogenous ALP mRNA	ALP-S (forward): TGG CCC CCA TGC TGA GTG ACA C ALP-AS (reverse): TGG CGC AGG GGC ACA GCA GAC	160	27
Endogenous GAPDH mRNA	GAPDH-S (forward): GGC GAT GCT GGC GCT GAG TA GAPDH-AS (reverse): ACA GTT TCC CGG AGG GGC CA	325	14
Exogenous OCT3/4 mRNA	βA-1 (forward): TCT GAC TGA CCG CGT TAC TCC CAC A OCT3/4-AS (reverse): GCT GAT CTG CTG CAG TGT GGG T	836	13,16
Exogenous SOX2 mRNA	βA-1 (forward): TCT GAC TGA CCG CGT TAC TCC CAC A SOX2-AS (reverse): GGC GCC GGG GAG ATA CAT GC	945	13,16
Transgene OCT3/4 DNA	β-gl-1S (forward): TGT GCT GTC TCA TCA TTT TGG CAA A OCT3/4-AS (reverse): GCT GAT CTG CTG CAG TGT GGG T	787	16,45
Transgene SOX2 DNA	β-gl-1S (forward): TGT GCT GTC TCA TCA TTT TGG CAA A SOX2-AS (reverse): GGC GCC GGG GAG ATA CAT GC	880	29,45

Exogenous octamer-binding transcription factor 3/4 (OCT3/4) and sex-determining region Y box 2 (SOX2) mRNA are derived from the transgenes pCXLE-hOCT3/4-shp53 and pCXLE-hSK, respectively. Similarly, exogenous OCT3/4 and SOX2 DNA correspond to the integrated pCXLE-hOCT3/4-shp53 and pCXLE-hSK in human deciduous tooth dental pulp cell-derived induced pluripotent stem cells (HDDPC-iPSCs), respectively. KLF4, Kruppel-like factor 4; ALP, alkaline phosphatase, tissue-nonspecific isozyme; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.