Figure 1.
Dispersed islet cells adhered on temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) dishes coated with various human laminins. Dispersed rat islet cells were plated at a density of 0.5 × 106 cells/cm2, and cell attachment status was assessed at day 1. Representative images of plated islet cells on PIPAAm dishes coated with (A) human laminin (HL)-332, (B) HL-511, and (C) HL-411. (D) Plating efficiencies expressed by the percentage of the number of attached islet cells against the total number of plated islet cells (n = 4). Scale bars: 100 μm. *p < 0.05 compared among the groups by one-way ANOVA with Scheffe’s least significant difference post hoc test.