Figure 3.

Insulin secretion of cultured islet cells in response to glucose stimulation. At day 3 of culture experiment, cells were incubated with 3.3 mmol/L glucose for 60 min as the first glucose treatment, 20 mmol/L glucose for 60 min as the second treatment, and 3.3 mmol/L glucose for 60 min as the final treatment (n = 4 for each coated surface). The columns (a) in three step treatments represent the amounts of secreted insulin from dispersed islet cells cultured on temperature-responsive polymer, poly (N-isopropylacrylamide) (PIPAAm) dishes (the negative control). The columns (b, c, d, e, and f) represent the amounts of secreted insulin from dispersed islet cells cultured on PIPAAm dishes coated with rat laninin-5 (the positive control), human laminin (HL)-211, HL-332, HL-411, and HL-511, respectively.