Table I.
List of primers and PCR conditions
| Primer no. | Gene | Primer sequence (5′-3′) | Annealing temperatures (°C) | Number of cycles |
|---|---|---|---|---|
| 1 | Actin | F=GCCTTCCTTCTTGGGTATGG | 55 | 32 |
| R=CAGCTCAGTAACAGTCCGC | ||||
| 2 | NP1 | F=CAGGACACTCTAGGTGGAGG | 55 | 32 |
| R=GAGGGGAGAAGAGAGACGAT | ||||
| 3 | NP2 | F=CATCAACGACAAGGTCGCAC | 55 | 32 |
| R=CTCTTCACAGGTCTCCACAGG | ||||
| 4 | NPR | F=CAAGAGCAGGATACCTTGGG | 55 | 32 |
| R=GAAGTGGGAGGTATAGCCAG | ||||
| 5 | IL-1β | F=TGAAGAAGAGCCCATCCTCTG | 55 | 32 |
| R=GGGTGTGCCGTCTTTCATTA |
NP1, neuronal pentraxin 1; NP2, neuronal pentraxin 2; NPR, neuronal pentraxin receptor; IL-1β, interleukin-1β; F, forward; R, reverse.