TABLE 1.

Primers used for pBD cDNA construction and for introducing mutations into the PB1 gene of the mutant viruses

Purpose	Primer(s) (5′–3′)a
	Forward	Reverse
PB2 amplification	CCAGCAAAAGCAGGTCAAATATATTCA	TTAGTAGAAACAAGGTCGTTTTTAAAT (DK/08), TTAGTAGAAACAAGGTCGTTTTTAAAC (CK/09)
PB1 amplification	CCAGCAAAAGCAGGCAAACCATTTGAATG	TTAGTAGAAACAAGGCATTTTTTCACG
PA amplification	CCAGCAAAAGCAGGTACTGATCCAAA	TTAGTAGAAACAAGGTACTTTTTTGGA
HA amplification	CCAGCAAAAGCAGGGGTTCACTCTGTC (DK/08), CCAGCAAAAGCAGGGGTCCAATCTGTC (CK/09)	TTAGTAGAAACAAGGGTGTTTTTAACTAC
NP amplification	CCAGCAAAAGCAGGGTAGATAATCAC	TTAGTAGAAACAAGGGTATTTTTCT
NA amplification	CCAGCAAAAGCAGGAGTTCA	TTAGTAGAAACAAGGAGT
M amplification	CCAGCAAAAGCAGGTAGATGT	TTAGTAGAAACAAGGTAGTTT
NS amplification	CCAGCAAAAGCAGGGTGACAAAAACAT	TTAGTAGAAACAAGGGTGTTTTTTA
CK/09 PB1D619N mutation	GAAATGGGAATTGATGGATGAAAACTACCAGGGCAG	CTGCCCTGGTAGTTTTCATCCATCAATTCCCATTTC
CK/09 PB1G622D mutation	GGATGAAGACTACCAGGACAGACTGTGCAATCCTC	GAGGATTGCACAGTCTGTCCTGGTAGTCTTCATCC
CK/09 PB1R635K mutation	CTGAATCCATTCGTCAGCCATAAGGAAATTGAATCTGTC	GACAGATTCAATTTCCTTATGGCTGACGAATGGATTCAG
CK/09 PB1D619N/G622D mutation	GGATGAAAACTACCAGGACAGACTGTGCAATCCTC	GAGGATTGCACAGTCTGTCCTGGTAGTTTTCATCC

^aThe nucleotides that have been changed are underlined and in boldface type.