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. 2016 Jan 28;90(4):1872–1879. doi: 10.1128/JVI.02387-15

TABLE 2.

Replication and lethality of H5N1 viruses in micea

Virus Virus replication on day 3 p.i. (log10 EID50 ± SD)b
MLD50 (log10 EID50)c Attenuation (fold)f
Lung Brain Spleen Kidney
DK/08 6.0 ± 0.5 6.2 NA
CK/09 6.7 ± 0.5 3.8 ± 0.6 4.2 ± 0.4 4.0 ± 0.7 1.6 NA
R-DK/08 6.0 ± 0.7 6.2 NA
R-CK/09 7.3 ± 0.1 4.5 ± 0.0 4.5 ± 0.0 4.4 ± 0.1 1.5 NA
CK/09-DK/08PB2 6.5 ± 0.7 4.5 ± 0.0 4.1 ± 0.7 4.4 ± 0.1 1.4 0
CK/09-DK/08PB1 5.3 ± 0.4d 1.5 ± 0.0d 4.5 1,000
CK/09-DK/08PA 6.5 ± 0.3 4.2 ± 0.4 4.2 ± 0.6 3.8 ± 0.7 1.2 0
CK/09-DK/08HA 6.8 ± 0.4 3.9 ± 0.5 4.5 ± 0.0 4.2 ± 0.6 1.3 0
CK/09-DK/08NP 6.6 ± 0.8 4.4 ± 0.1 4.4 ± 0.1 4.3 ± 0.1 1.2 0
CK/09-DK/08NA 7.1 ± 0.5 4.5 ± 0.0 4.4 ± 0.1 4.5 ± 0.0 1.2 0
CK/09-DK/08 M 6.8 ± 0.6 3.4 ± 0.8 4.4 ± 0.1 4.1 ± 0.5 1.4 0
CK/09-DK/08NS 6.7 ± 0.3 4.1 ± 0.5 4.5 ± 0.0 4.0 ± 0.7 1.2 0
Chimera 1 6.3 ± 0.4 3.5 ± 0.9 4.4 ± 0.1 4.2 ± 0.6 1.8 2
Chimera 2 4.9 ± 0.3d 1.4 ± 1.0d 1.3 ± 0.9d 4.5 1,000
CK/09-PB1D619N 6.6 ± 0.1 2.9 ± 1.4 4.0 ± 0.4 3.8 ± 0.4 1.4 0
CK/09-PB1G622D 5.8 ± 0.5d 1.2 ± 0.8d 2.9 ± 2.1d 2.0 ± 1.3d 4.2 501
CK/09-PB1R635K 6.5 ± 0.2 3.3 ± 0.9 3.6 ± 0.6 3.6 ± 0.9 2.5 10
CK/09-PB1D619N+G622D 5.3 ± 0.4 2.3 ± 0.3d 4.5 1,000
CK/09-PB1G622D+R635K 3.8 ± 0.6e 3.3 ± 0.5 1.3 ± 1.0e 4.2 501
a

Six-week-old female BALB/c mice were used for these studies.

b

Groups of three mice were inoculated intranasally with 106 EID50 of the test virus in a 50-μl volume and were killed on day 3 postinoculation (p.i.); organs were then collected for virus titration in eggs. −, no virus was detected in undiluted samples. Virus titers of mice were compared by using the Student-Newman-Keuls test. SD, standard deviation.

c

The 50% mouse lethal dose (MLD50) was determined by intranasally inoculating groups of five mice with 10-fold serial dilutions containing 101 to 106 EID50 of virus in a 50-μl volume.

d

P < 0.05 compared with the titers in the corresponding organs of the R-CK/09-inoculated mice.

e

P < 0.01 compared with the titers in the corresponding organs of the R-CK/09-inoculated mice.

f

Virulence decrease compared with the R-CK/09 virus. NA, not applicable.