FIG 1.

Key domains of pUS9 are conserved across the Alphaherpesvirinae subfamily. pUS9 is a type II transmembrane protein consisting of an acidic domain and a basic domain within the cytoplasmic tail, in addition to a transmembrane (TM) domain and a short C-terminal extracellular domain (ED). The encoded pUS9 sequences were obtained from the following herpesviral genome sequence accession numbers: HSV-1 strain 17, X14112; HSV-1 strain F, GU734771; HSV-2, Z86099; simian agent 8 (SA8), AY714813; herpesvirus B (HVB), AF533768; BHV-1, AJ004801; BHV-5, AY261359; PrV, BK001744; EHV-1, AY665713; equine herpesvirus 4 (EHV-4), AF030027; and VZV, X04370. Clustal W alignments were generated using Lasergene (version 11.1) MegAlign from DNAStar. The kinesin-1 binding domain (residues 56 to 60 are underlined for HSV-1) determined in this study maps within the basic domain of pUS9. The consensus residue is shown above the alignments when at least 5 residues of one type only match between aligned sequences; otherwise, residues are indicated by dots or dashes. Consensus strength is shown above the alignments as vertical bars (red, 11 residues match; orange, 9 residues match; green, 7 residues match; light blue, 5 residues match; dark blue, <5 residues match).