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Characterization of HSV-1pUS9KBDM and HSV-1pUS9KBDR (rescuant), generated by BAC recombination. Vero cell lysates harvested at 24 h p.i. and immunoblotted with the indicated antibodies are shown. Relative to those of HSV-1Fparental (F strain), HSV-1pUS9KBDM and HSV-1pUS9KBDR showed similar expression of major viral structural proteins. Only in the case of HSV-1pUS9KBDM was there enhanced expression of pUS9.
