TABLE 1.
Oligonucleotides used for construction of recombinant viruses and for qPCR of DNA from ganglia
| Name | Sequencea |
|---|---|
| US9galK-Forward | CGGCCAACGACTTCCTCGTACGCATGGGCCGCCAACAGTCGGTATTAAGGCCTGTTGACAATTAATCATCGGCA |
| US9galK-Reverse | GACAGAACGGCCACGAGGAGACAGGCGATCACCATGCCGACGCAGCGGGTTCAGCACTGTCCTGCTCCTT |
| US9KBDM-Forward | CGGCCAACGACTTCCTCGTACGCATGGGCCGCCAACAGTCGGTATTAAGGAATAACAACAATAACACCCGCTGCGTCGGCATGGTGATCGCCTGTCTCCTCGTGGCCGTTCTGTC |
| US9KBDM-Reverse | GACAGAACGGCCACGAGGAGACAGGCGATCACCATGCCGACGCAGCGGGTGTTATTGTTGTTATTCCTTAATACCGACTGTTGGCGGCCCATGCGTACGAGGAAGTCGTTGGCCG |
| US9KBDR-Forward | CGGCCAACGACTTCCTCGTACGCATGGGCCGCCAACAGTCGGTATTAAGGCGTCGACGCAGACGCACCCGCTGCGTCGGCATGGTGATCGCCTGTCTCCTCGTGGCCGTTCTGTC |
| US9KBDR-Reverse | GACAGAACGGCCACGAGGAGACAGGCGATCACCATGCCGACGCAGCGGGTGCGTCTGCGTCGACGCCTTAATACCGACTGTTGGCGGCCCATGCGTACGAGGAAGTCGTTGGCCG |
| UL35-Forward | GTCTTGGCCACCAATAACTC |
| UL35-Reverse | GGGTAAACGTGTTGTTTGCG |
| mGAPDH-Forward | GATGGGTGTGAACCACGAG |
| mGAPDH-Reverse | GTGATGGCATGGACTGTGG |
a
Homology arms for BAC recombination are underlined.