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. 2016 Jan 28;90(4):1812–1823. doi: 10.1128/JVI.02599-15

FIG 1.

FIG 1

Influenza A virus induces a dose-dependent increase in platelet-endothelial adhesion. Endothelial monolayers infected with increasing MOIs of influenza (Flu 1, MOI of 1; Flu 5, MOI of 5, etc.) have increased platelet adhesion, whether infected with a laboratory strain of H3N2 (A) (n = 5 to 7), a clinical H3N2 strain (B) (n = 3), or a laboratory strain of H1N1 (C) (n = 3). *, P < 0.05 versus control. (D to E) Influenza virus induces a dose-dependent increase in platelet-endothelial interaction in studies using endothelial cells in suspension. (D) Endothelial cells bound to platelets appear in the upper right quadrant. (E) Quantification of data from panel D. n = 3; *, P < 0.05 versus the control. (F) Influenza virus-induced platelet-endothelial interaction visualized by confocal microscopy. Calcein-AM-labeled platelets are shown in green, while endothelial cell-cell junctions stained with VE-cadherin appear in red. The image is representative of 2 experiments.