TABLE 2.
Percent change of the RIG-I-like interferon antiviral pathway upon RSV-A2 infection and treatment with antiviral compounds in A549 and primary HAE cells determined by RT-qPCR
| Gene | Cell linea | % change of the RIG-I-like interferon antiviral pathway |
|||
|---|---|---|---|---|---|
| TMC-353121 | RSV-604 | YM-53403 | BI-D | ||
| RSV Nb | A549 | 41 ± 4 | 44 ± 8 | 11 ± 2 | 4 ± 0.4 |
| HAE donor 1 | 48 ± 9 | 1.6 ± 0.1 | 18 ± 2 | 14 ± 10 | |
| HAE donor 2 | 62 ± 6 | 27 ± 12 | 34 ± 10 | 34 ± 11 | |
| RIG-I | A549 | 103 ± 26 | 61 ± 28 | 39 ± 2 | 183 ± 49 |
| HAE donor 1 | 103 ± 8 | 35 ± 5 | 82 ± 4 | 639 ± 103 | |
| HAE donor 2 | 135 ± 36 | 162 ± 34 | 154 ± 16 | 924 ± 50 | |
| IFN-β1 | A549 | 30 ± 10 | 14 ± 4 | 9 ± 2 | 435 ± 123 |
| HAE donor 1 | 292 ± 24 | 205 ± 45 | 273 ± 11 | 741 ± 232 | |
| HAE donor 2 | 88 ± 3 | 89 ± 29 | 103 ± 10 | 286 ± 13 | |
| MX2 | A549 | 188 ± 20 | 84 ± 21 | 59 ± 7 | 207 ± 68 |
| HAE donor 1 | 59 ± 7 | 12 ± 2 | 36 ± 14 | 425 ± 40 | |
| HAE donor 2 | 65 ± 25 | 81 ± 17 | 74 ± 6 | 596 ± 28 | |
a
HAE cell analysis was performed with 3 donors, with each experiment performed in triplicate. The infection of the third donor was insufficient to provoke a response. A549 data were averaged from the results of 2 independent experiments.
bGene data are expressed as a percentage of the results determined for each donor or untreated A549 cell DMSO infection (assigned a value of 100%)