TABLE 3.
Fold changes in antiviral pathway mRNA transcript counts upon RSV-A2 infection and treatment with antiviral compounds in A549 cellsa
| Product category and gene | Fold change in antiviral pathway mRNA transcript counts |
||||
|---|---|---|---|---|---|
| DMSO | TMC-353121 | RSV-604 | YM-53403 | BI-D | |
| Pattern recognition receptors | |||||
| DDX58 (RIG-I) | 37 | 51 | 33 | 23 | 166 |
| IFIH1 (MDA-5) | 155 | 191 | 120 | 87 | 724 |
| DHX58 (LGP2) | 101 | 82 | 45 | 63 | 349 |
| TLR3 | 12 | 30 | 20 | 11 | 48 |
| TLR4 | 1 | −2 | −2 | 3 | 5 |
| TLR7 | 1 | 2 | 1 | −1 | 2 |
| TLR9 | 2 | 2 | 2 | 1 | 1 |
| Mitochondrial signaling | |||||
| MAVS | −1 | −1 | 1 | −1 | −1 |
| TMEM173 (Sting) | 10 | 8 | 2 | 6 | 17 |
| TRAP | −2 | −1 | −2 | −1 | −2 |
| TBK1 | −1 | 1 | −1 | −1 | 1 |
| IKBKG (Nemo) | −1 | 1 | 1 | 1 | 1 |
| Transcription factors | |||||
| IRF1 | 11 | 10 | 6 | 6 | 21 |
| IRF3 | −1 | 1 | −1 | 1 | 1 |
| IRF7 | 27 | 62 | 57 | 27 | 60 |
| IRF9 | 24 | 33 | 32 | 23 | 53 |
| NFKB1 | 2 | 2 | 2 | 2 | 3 |
| NFKB2 | 5 | 4 | 4 | 3 | 7 |
| Type I, II, and III interferons | |||||
| IFN-α1 | 1 | 1 | 1 | 1 | 31 |
| IFN-α10 | 1 | 1 | 1 | 1 | 10 |
| IFN-α13 | 6 | 1 | 1 | 1 | 42 |
| IFN-α7 | 1 | 1 | 1 | 1 | 16 |
| IFN-β1 | 130 | 42 | 25 | 18 | 1,753 |
| IL-6 | 508 | 159 | 66 | 55 | 954 |
| IFN-γ | 1 | 1 | 1 | 1 | 13 |
| IL28A | 383 | 239 | 48 | 74 | 2,161 |
| IL28B | 202 | 119 | 23 | 43 | 954 |
| IL-29 | 1,778 | 647 | 265 | 259 | 6,013 |
| ISGs | |||||
| CCL5 (RANTES) | 147 | 67 | 51 | 87 | 1,168 |
| CX3CL1 (Fractalkine) | 16 | 18 | 11 | 5 | 54 |
| CXCL10 (IP-10) | 253 | 196 | 62 | 97 | 3,069 |
| CXCL11 | 321 | 187 | 86 | 145 | 2,357 |
| IFI27 | 443 | 855 | 671 | 551 | 1,349 |
| IFI44 | 1,695 | 2,703 | 1,469 | 1,127 | 4,487 |
| IFIT2 | 387 | 355 | 232 | 217 | 3,005 |
| IFIT3 | 159 | 220 | 118 | 116 | 761 |
| IFITM1 | 345 | 862 | 258 | 418 | 1,312 |
| ISG15 | 142 | 310 | 277 | 193 | 445 |
| ISG20 | 19 | 19 | 17 | 10 | 54 |
| OAS1 | 9 | 19 | 12 | 9 | 33 |
| OAS2 | 1,041 | 2,282 | 1,075 | 988 | 3,183 |
| OAS3 | 8 | 15 | 9 | 8 | 19 |
| OASL | 754 | 713 | 602 | 562 | 4,339 |
| MX1 | 257 | 650 | 302 | 234 | 861 |
| MX2 | 102 | 337 | 130 | 84 | 415 |
| RSAD2 (Viperin) | 228 | 211 | 140 | 57 | 2,170 |
| TSLP | 4 | 1 | 1 | 1 | 16 |
a
Data are from the results of one RNA sequencing experiment. Selected genes were validated by RT-qPCR (Table 2) and protein expression as described in Results. ISGs, interferon-stimulated genes.