FIG 6.
Oligomeric status of SP1-Zip. (A) Estimation of Rh of SP1-Zip by QELS. Shown are the QELS fit, the raw autocorrelation data, and root mean square deviation of the fit. (B) Rubpy-mediated photo-cross-linking in SP1-Zip oligomers. SP1-Zip containing a His6 tag was irradiated in the presence of Rubpy for different intervals, as described in Materials and Methods, and then analyzed by SDS-PAGE. Lanes: 1, MW markers (in thousands); 2, irradiation for 0 s; 3, irradiation for 20 s; 4, irradiation for 2 min; 5, irradiation for 2 min in the presence of 10× Rubpy. (C) SEC-MALS of SP1-Zip bundles. Shown are the elution chromatograms for SP1-Zip, ovalbumin (OVA) (∼43 kDa), and carbonic anhydrase (CA) (∼29 kDa).