FIG 4.
Enhanced EV71 replication following silencing of host virus resistance genes. (A) Fold change of EV71 titers in Vero cells upon silencing of top 11 hits of genes selected from the poliovirus screen. Values were calculated relative to the average titers in cells transfected with the nontargeting control siRNA (NTC) (n = 5). Cells transfected with an EV71-targeting siRNA (siEV71) were included as controls. Data represent the average fold increases in three transfection experiments ± SEM. (B) Plaque assays were performed on 10−5 dilutions of EV71 supernatants collected from siRNA-transfected Vero cells.