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. 2016 Jan 28;90(4):1694–1704. doi: 10.1128/JVI.01464-15

FIG 7.

FIG 7

Effects of ZNF205 and EP300 gene silencing on programmed cell death. (A) Expression levels of HMGB1 (necrosis marker), ZNF205, and EP300 in ZNF205- and EP300-silenced Vero cells infected with Sabin-1 virus. Expression levels were calculated relative to Sabin-1-infected cells transfected with siNTC. Data represent mean values of four transfection experiments ± SEM. (B) Sabin-1 poliovirus titers in Vero cells cultured in the presence of 33 μM Ac-DEVD-CHO or 33 μM necrostatin-1 (inhibitor for the apoptotic pathway or the necrotic pathway, respectively). Data represent the average titers from four experiments; for mock infection experiments, n = 10. Error bars indicate SEM. (C) Fold changes in expression levels of genes of the apoptotic pathway in Sabin-1-infected Vero cells upon ZNF205 and EP300 silencing. Values are expressed relative to expression levels in Sabin-1-infected Vero cells transfected with the siNTC. Data represent average values of four transfection experiments. Only data for genes for which expression was altered by ZNF205 and/or EP300 silencing are presented.