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. 2016 Jan 28;90(4):1931–1943. doi: 10.1128/JVI.02392-15

FIG 2.

FIG 2

Nek9 affects viral gene expression. (A) IMR-90 cells that were transfected with siRNAs, either siControl as a control or siNek9 to deplete Nek9, were infected with dl309 24 h after knockdown at an MOI of 5. Viral gene expression was monitored by RT-qPCR at the indicated time. GAPDH mRNA was used as the normalization control. Insets show cellular and viral protein expression at 16 and 48 h after viral infection in control or Nek9-depleted cells after resolving 20 μg of total protein from infected IMR-90 cells. n = 4; error bars represent SD. (B) MEF/3T3-Nek9V5 cells were induced to express Nek9 by removal of doxycycline as described in Materials and Methods. Cells were infected with dl309 at an MOI of 5 24 h after Nek9 induction. Viral gene expression was monitored by RT-qPCR as described for panel A with mouse GAPDH mRNA as the normalization control. The inset shows Nek9 induction 24 h after doxycycline removal from the cell culture medium. n = 3; error bars represent SD.