FIG 7.

Formation of virion-containing vesicles during KSHV lytic replication. (A and B) iSLK.BAC16 cells were induced with doxycycline and sodium butyrate. Cells were harvested at 60 h postinduction and osmotically disrupted, left untreated (nontreated; NT), or treated with 0.5% Triton X-100, NP-40, or SDS for 30 min on ice and then subjected to a membrane flotation assay. Six continuous fractions were collected. The viral components in each fraction were analyzed by Western blotting using the indicated antibodies (A), and the viral DNA in each fraction was determined by qPCR (B). The numbers in the top left of each graph (Fr1% and Total) indicate the percentages of viral DNA detected in the first fraction relative to the total viral DNA and the total viral DNA of all fractions, respectively.