FIG 9.

ORF33 and ORF38 are required for vesicle transport of virions. (A and B) iSLK.BAC16 cells were induced with doxycycline and sodium butyrate. Cells were harvested at 60 h postinduction, osmotically disrupted, and then subjected to a membrane flotation assay. Six continuous fractions were collected. The viral components in each fraction were analyzed by Western blotting using the indicated antibodies (A), and the viral DNA of each fraction was determined by qPCR (B). The numbers in the top left of each graph (Fr1%) indicate the percentages of viral DNA detected in the first fraction relative to the total viral DNA.