FIG 8.

Quantitative immuno-EM confirms that MHR mutations arrest Gag after PM targeting. Immuno-EM images of infected COS-1 cells described in Fig. 7 were analyzed quantitatively (Table 1). For the indicated constructs, graphs show the average number of Gag clusters in 10 μm of PM (A) and the number of targeted Gag clusters, early assembly sites, and late assembly sites at the PM as a percentage of total Gag clusters at the PM (B). Targeted, early, and late sites are defined in the text and in Table 1. Error bars show standard errors of the means from two independent experiments. Brackets indicate two comparisons that were either not significant (NS) or significant (P < 0.002) using the unpaired Student t test.