FIG 1.
Synergetic inhibition of FIPV Mpro by Zn2+ and N3. (A) Inhibition of FIPV Mpro by different compounds. Fluorescence curve of FIPV Mpro free of inhibitors (black) or with 1 μM N3 (orange), 2 μM Zn2+ (green), or the dual inhibitors (blue), respectively. The fluorescence intensity is plotted against time to represent enzyme activity. (B and C) Secondary plots to determine the kinetic constants (αKi and Ki) of Zn2+ as a noncompetitive inhibitor. The values of αKi (B) and Ki (C) are calculated from the x intercept.