FIG 3.

EIAV MA determines Gag targeting to internal cellular membranes. (A) The MA swap between EIAV and HIV-1 impaired the release of chimeric Gag. Schematic structures of chimeric Gags were shown. Wild-type Gag and HMA-EGag and EMA-HGag fused with green fluorescent protein (GFP) in the C terminus were transfected into 293T cells. Expression and release efficiency were detected by Western blotting as in Fig. 2B. (B) 293T and ED cells were transfected with wild-type and chimeric Gag. The cellular localization of Gag was analyzed by confocal microscopy.